Adenovirus Real Time PCR Kit

Adenovirus Real Time PCR Kit

  • Adenovirus Real Time PCR Kit
Model No.: JC10117
Min. Order: 5 Pack/Packs
Payment Type: T/T,L/C,D/A,D/P
Transportation: Air
Delivery Time: 7 Days
Place of Origin: China
Brand: Bioperfectus
Certificate: ISO13485

Product Description

Jiangsu Bioperfectus Technologies®


Company profile

Jiangsu Bioperfectus Technologies is a professional innovative manufacturing enterprise that is oriented in molecular diagnostic techniques. Since established in 2010, Jiangsu Bioperfectus Technologies has helped improve productivity and efficiency for doctors and laboratory researchers worldwide by delivering innovative products and services backed by the highest levels of quality and expertise. We believe our products also provide economic benefits for payers.

Of Bioperfectus`s team, one is entitled into "The Recruitment Program of Global Experts" as distinguished expert; three are rewarded as [Double Gen Talent Person". The company was awarded as "The most potential oversea returnee entrepreneurial companies in 2013". It strives to integrate quality, efficiency, fairness and impartiality into its core culture, and provide the best products and services.


Adenovirus Real Time PCR Kit


1. Intended use

The Bioperfectus Technologies® Adenovirus Real Time PCR Kit is used for the detection of Adenovirus by using real time PCR systems. The Adenovirus Real Time PCR Kit contains 4 types of super mix for the specific amplification of the virus RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription(RT), during which the virus RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of PCR (Polymerase chain reaction). Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. The detection of amplified virus DNA fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. An external positive control is supplied which allow the determination of the gene load.


Workflow

DNA Extraction-PCR amplification-Analyze result


2. Kit components

Components

Number of Vials

Volume (µl/Vial)

PCR Master Mix

1

375

PCR Enzyme Mix

1

250

Reaction Mix

1

200

Positive Control

1

300

Internal Control

1

500

PCR Grade Water

1

500

*NOTE: Components from different batch number kit can`t be used interchangeably.


3. Storage

l The Bioperfectus Technologies® Adenovirus Real Time PCR Kit is shipped on dry ice. The components of the kit should arrive frozen. If components are not frozen upon receipt, contact Bioperfectus Technologies for assistance.

l All reagents should be stored at-20℃ until the expiration date listed on the outer kit box. Storage at +4℃ above should not be recommended.

l Protect Reaction Mix from light during storage.

Repeated freezing and thawing of reagents (more than five times) should be avoided, as this might affect the performance of the assay. The reagents should be frozen in aliquots, if they are to be used intermittently.


4. Material and Devices Required but Not Provided

l Appropriate real-time PCR instrument: ABI7500, Roche Lightcycler 480

l Appropriate nucleic acid extraction system or kit

l Desktop centrifuge with a rotor for 2 ml reaction tubes

l Microcentrifuge with a rotor for 0.2 ml reaction tubes

l Vortex mixer

l Pipettes (adjustable)

l Pipette tips with filters (disposable)

l Powder-free gloves (disposable)

*NOTE: Please ensure that instruments have been installed, calibrated, checked and maintained according to the manufacturer`s instructions and recommendations.


5. Specimens type

Upper respiratory tract:

  • Nasal swab
  • Nasopharyngeal aspirate
  • Nasopharyngeal swab
  • Throat swab

lower respiratory tract:

  • Transtracheal aspirate
  • Lung biopsy
  • Bronchoalveoar lavage
  • Post-mortem lung or tracheal tissue

For laboratory diagnosis:

  • Nasopharyngeal aspirate
  • Acute serum
  • Convalescent serum


6. Features and Benifits

  • Easy: Easy reaction setup and result analysis
  • Reliable: Provides accurate results from one-step RT-PCR reaction
  • Fast: Turnaround time of 3 hours from sample to result
  • Clear: Provides clear results that fulfill WHO parameters for surveillance and monitoring of Influenza outbrea

  • Detection and quantification of human Adenovirus (HAdV) specific DNA
  • Detection of all known human pathogen Adenovirus species
  • Ready-to-use kit including Internal Control and Quantification Standards
  • Compatible with various Real-Time PCR platforms
  • CE-IVD marked in-vitro diagnostic test

For more information about Bioperfectus Technologies, please visit our web-site or contact us via email.

If you have questions, please feel free to contact us.

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