Dengue Virus Real Time PCR Kit

Dengue Virus Real Time PCR Kit

  • Dengue Virus Real Time PCR Kit
Model No.: JC30101
Min. Order: 5 Pack/Packs
Payment Type: T/T,L/C,D/A,D/P
Transportation: Air
Productivity: KANBAN
Delivery Time: 7 Days
Place of Origin: China
Brand: Bioperfectus
Certificate: ISO13485

Product Description

Jiangsu Bioperfectus Technologies®

Company profile

Jiangsu Bioperfectus Technologies is a professional innovative manufacturing enterprise that is oriented in molecular diagnostic techniques. Since established in 2010, Jiangsu Bioperfectus Technologies has helped improve productivity and efficiency for doctors and laboratory researchers worldwide by delivering innovative products and services backed by the highest levels of quality and expertise. We believe our products also provide economic benefits for payers.

Of Bioperfectus`s team, one is entitled into "The Recruitment Program of Global Experts" as distinguished expert; three are rewarded as [Double Gen Talent Person". The company was awarded as "The most potential oversea returnee entrepreneurial companies in 2013". It strives to integrate quality, efficiency, fairness and impartiality into its core culture, and provide the best products and services.

Dengue Virus Real Time PCR Kit CE-Marked

1. Intended use

Dengue is a single-stranded RNA virus which belongs to the family of Flavi viruses. Aedes mosquitoes are responsible for transmission to humans and thus the disease affects mostly urban regions with tropical or subtropical climate. About 80% of all Dengue infections are asymptomatic while most others have only fever, headaches and skin rush and the disease may take a few weeks. However, a second infection with a different type can be fatal, making the Dengue virus dangerous.

The Bioperfectus Technologies® Dengue Virus Real Time PCR Kit provides a fast, easy and accurate system to identify this target in a nucleic acid extract. A control amplification reaction acts as internal control (IC). The resulting PCR product can be applied directly on a Chipron DNA-Array to determine the type of Dengue virus. The kit is used for the detection of Streptococcus pyogenes in samples like nasal and pharyngeal secretions, sputum, provoked sputum, bronchial lavage and etc. The kit is test


DNA Extraction-PCR amplification-Analyze result

2. Kit components


Number of Vials

Volume (µl/Vial)

PCR Master Mix



PCR Enzyme Mix



Reaction Mix



Positive Control



Internal Control



PCR Grade Water



*NOTE: Components from different batch number kit can`t be used interchangeably.

3. Storage

l The Bioperfectus Technologies® Dengue Virus Real Time PCR Kit is shipped on dry ice. The components of the kit should arrive frozen. If components are not frozen upon receipt, contact Bioperfectus Technologies for assistance.

l All reagents should be stored at-20℃ until the expiration date listed on the outer kit box. Storage at +4℃ above should not be recommended.

l Protect Reaction Mix from light during storage.

Repeated freezing and thawing of reagents (more than five times) should be avoided, as this might affect the performance of the assay. The reagents should be frozen in aliquots, if they are to be used intermittently.

4. Material and Devices Required but Not Provided

l Appropriate real-time PCR instrument: ABI7500, Roche Lightcycler 480

l Appropriate nucleic acid extraction system or kit

l Desktop centrifuge with a rotor for 2 ml reaction tubes

l Microcentrifuge with a rotor for 0.2 ml reaction tubes

l Vortex mixer

l Pipettes (adjustable)

l Pipette tips with filters (disposable)

l Powder-free gloves (disposable)

*NOTE: Please ensure that instruments have been installed, calibrated, checked and maintained according to the manufacturer`s instructions and recommendations.

5. Specimens type

Upper respiratory tract:

  • Nasal swab
  • Nasopharyngeal aspirate
  • Nasopharyngeal swab
  • Throat swab

lower respiratory tract:

  • Transtracheal aspirate
  • Lung biopsy
  • Bronchoalveoar lavage
  • Post-mortem lung or tracheal tissue

For laboratory diagnosis:

  • Nasopharyngeal aspirate
  • Acute serum
  • Convalescent serum

6. Features and Benifits

  • Easy: Easy reaction setup and result analysis
  • Reliable: Provides accurate results from one-step RT-PCR reaction
  • Fast: Turnaround time of 3 hours from sample to result
  • Flexibility – Presence/absence or threshold testing available
  • Accuracy - Automated DNA-based analysis instead of subjective plate counts

7. Designed for efficient workflow and reliable results

  • 50 tests per kit
  • Lysis reagents included
  • Step-by-step directions included in each package

8. Associated Kits

Cat. No. Product Discription Packaging
Dengue Virus Real Time PCR Kit CE-MARKED
Dengue Virus Type 1 Real Time PCR Kit
Dengue Virus Type 2 Real Time PCR Kit
Dengue Virus Type 3 Real Time PCR Kit
Dengue Virus Type 4 Real Time PCR Kit

For more information about Bioperfectus Technologies, please visit our web-site or contact us via email.

If you have questions, please feel free to contact us.

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