Haemophilus Influenzae Real Time PCR Kit

Haemophilus Influenzae Real Time PCR Kit

  • Haemophilus Influenzae Real Time PCR Kit
Model No.: JB20104
Min. Order: 5 Pack/Packs
Payment Type: T/T,L/C,D/A,D/P
Transportation: Air
Productivity: KANBAN
Delivery Time: 7 Days
Place of Origin: China
Brand: Bioperfectus
Certificate: ISO13485

Product Description

Jiangsu Bioperfectus Technologies®


Company profile

Jiangsu Bioperfectus Technologies is a professional innovative manufacturing enterprise that is oriented in molecular diagnostic techniques. Since established in 2010, Jiangsu Bioperfectus Technologies has helped improve productivity and efficiency for doctors and laboratory researchers worldwide by delivering innovative products and services backed by the highest levels of quality and expertise. We believe our products also provide economic benefits for payers.

Of Bioperfectus`s team, one is entitled into "The Recruitment Program of Global Experts" as distinguished expert; three are rewarded as [Double Gen Talent Person". The company was awarded as "The most potential oversea returnee entrepreneurial companies in 2013". It strives to integrate quality, efficiency, fairness and impartiality into its core culture, and provide the best products and services.


Haemophilus Influenzae Real Time PCR Kit


1. Intended use

The Bioperfectus Technologies® Haemophilus Influenzae Real Time PCR Kit is a research tool used for the detection of virulence factors from H.influenzae. This multi-assay kit provides microbiology researchers with a convenient way to quickly detect virulence factor genes from H.influenzae in isolated bacterial colonies, metagenomic samples, or other sample types. The kit contains assays for detection H.influenzae itself and the virulence factor genes hap and tbpA. The assays use PCR amplification primers and hydrolysis-probe detection, increasing their specificity. The kit also contains Microbial qPCR Mastermix, Microbial DNA-Free Water, Microbial DNA Positive Control, and Positive PCR Control. The positive PCR Control assay is included to test for the presence of PCR inhibitors and the efficiency of the polymerase chain reaction. Identification of whether pathogenic H. influenzae is present in a sample and what virulence factors may be involved in its pathogenicity is accomplished using data analysis software with imported CT values generated by the PCR instrument software. The simplicity of the product format and operating procedure allow routine and reliable screening of pathogenic H.influenzae from samples in any research laboratory with access to a real-time PCR instrument.


Workflow

DNA Extraction-PCR amplification-Analyze result


2. Kit components

Components

Number of Vials

Volume (µl/Vial)

PCR Master Mix

1

375

PCR Enzyme Mix

1

250

Reaction Mix

1

200

Positive Control

1

300

Internal Control

1

500

PCR Grade Water

1

500

*NOTE: Components from different batch number kit can`t be used interchangeably.


3. Storage

l The Bioperfectus Technologies® Haemophilus Influenzae Real Time PCR Kit is shipped on dry ice. The components of the kit should arrive frozen. If components are not frozen upon receipt, contact Bioperfectus Technologies for assistance.

l All reagents should be stored at-20℃ until the expiration date listed on the outer kit box. Storage at +4℃ above should not be recommended.

l Protect Reaction Mix from light during storage.

Repeated freezing and thawing of reagents (more than five times) should be avoided, as this might affect the performance of the assay. The reagents should be frozen in aliquots, if they are to be used intermittently.


4. Material and Devices Required but Not Provided

l Appropriate real-time PCR instrument: ABI7500, Roche Lightcycler 480

l Appropriate nucleic acid extraction system or kit

l Desktop centrifuge with a rotor for 2 ml reaction tubes

l Microcentrifuge with a rotor for 0.2 ml reaction tubes

l Vortex mixer

l Pipettes (adjustable)

l Pipette tips with filters (disposable)

l Powder-free gloves (disposable)

*NOTE: Please ensure that instruments have been installed, calibrated, checked and maintained according to the manufacturer`s instructions and recommendations.


5. Specimens type

Upper respiratory tract:

  • Nasal swab
  • Nasopharyngeal aspirate
  • Nasopharyngeal swab
  • Throat swab

lower respiratory tract:

  • Transtracheal aspirate
  • Lung biopsy
  • Bronchoalveoar lavage
  • Post-mortem lung or tracheal tissue

For laboratory diagnosis:

  • Nasopharyngeal aspirate
  • Acute serum
  • Convalescent serum


6. Features and Benifits

  • Easy: Easy reaction setup and result analysis
  • Reliable: Provides accurate results from one-step RT-PCR reaction
  • Fast: Turnaround time of 3 hours from sample to result
  • Flexibility – Presence/absence or threshold testing available
  • Accuracy - Automated DNA-based analysis instead of subjective plate counts


7. Designed for efficient workflow and reliable results

  • 50 tests per kit
  • Lysis reagents included
  • Step-by-step directions included in each package

8. Associated Kits

Cat. No.
Product discription
Packaging
JB20104
Haemophilus Influenzae Real Time PCR Kit
50T
JB20104-1
Haemophilus Influenzae B Real Time PCR Kit
50T




For more information about Bioperfectus Technologies, please visit our web-site or contact us via email.

If you have questions, please feel free to contact us.

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